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Green Aracari Genetic Analysis Project

Evaluation of genetic relatedness among green aracaris and an evaluation of the utility of DNA "fingerprint" data for use in maximizing gene diversity

Principal Investigator (PI): Kevin Willis, Biological Programs Director, Minnesota Zoo

This project has two objectives: 1) To evaluate levels of genetic relatedness among green aracaris in the North American captive population; and 2) To evaluate the utility of DNA "fingerprint" data in attempting to maximize gene diversity in captive populations.

The green aracari is the second most numerous Ramphastid held in captivity, and yet the long-term viability of this population is uncertain primarily because of a lack of pedigree information. This is a situation in which both wild-caught and captive born birds have passed through a single facility, and thus the current method of dealing with unknowns (Willis, 1993)* is not necessarily applicable. If all birds of unknown ancestry are excluded from analyses, almost one half of the current population is eliminated. Long-term viability of this population may very well hinge on resolution of genetic relationships.

DNA "fingerprint" technology has been touted as the solution to resolving genetic relationships among animals; however, there has not been an evaluation of the costs and benefits of using the data relative to population management goals. Even if estimates of relatedness among individuals are unbiased, the costs of over- and underestimating relatedness are not equal (Willis, 1993) and so using the data can decrease managed gene diversity. In this study data from birds of known relatedness will be used to develop a standard that will then be applied to birds of unknown relatedness. From this a cost-benefit analysis can be used to determine the best way to integrate this information into a management scheme.


*Willis, K. 1993. Use of animals of unknown ancestries in scientifically managed breeding programs. Zoo Biology, 12:161-172.