Semen Collection and Storage, Estrus Evaluation and Assisted Reproduction in Mexican Gray Wolves, SSP Population

To optimize genetic security of the Mexican gray wolf population, it’s essential that reproductive samples be collected and preserved. Objectives of this Mexican Wolf SSP three-year Action Plan priority project is to continue to collect and preserve Mexican gray wolf semen, evaluate current semen preservation techniques, determine whether vaginal cytology or fecal samples can be used to document ovulation or onset of estrus, determine whether male behavioral changes can be used to document initial rise in progesterone in females, correlate courtship and mating behavior with fecal hormones, and lastly to utilize information gathered in the above objectives to attempt artificial insemination in targeted individuals.

This project constitutes an important step in the systematic evaluation of current semen freezing programs and should significantly advance our knowledge of potential markers of estrus and ovulation. Ultimately, this information will allow us to plan strategies which can best insure the genetic security of the Mexican gray wolf. Refining techniques for cryopreservation of semen, determining female ovulatory patterns and the optimum time for artificial insemination could ultimately be important to the exchange of reproductive materials between captive and wild populations in the U.S. and Mexico.

The Ulysses S. Seal Conservation Grant Program awarded this project conservation project $1000. Staff Champion for the project was Jackie Fallon, Northern Trail Keeper.